Translational_Unit

Part:BBa_M36428:Design

Designed by: Anna Jaffe   Group: Stanford BIOE44 - S11   (2014-10-23)

RBS, spacer, Bxb1 integrase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1447
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1447
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1447
    Illegal AgeI site found at 1501
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has a strong E. coli RBS followed by a 6-bp spacer and the sequence for the Bxb1 integrase.

Source

The Bxb1 integrase from the cited paper was used as the gene in this construct, the spacer is an arbitrary sequence of 6 base pairs, and the RBS is a strong E.coli RBS found on the parts registry.

References

Bonnet J, Subsoontorn P, Endy D. 2014. Rewritable Digital Data Storage In Live Cells Via Engineered Control Of Recombination Directionality. PNAS. 109(23): 8884-8889.